Sentences with word «hepatocyte»

Binding and uptake of radiolabeled ApoC - III — depleted TRLs was significantly increased in hepatocytes isolated from Ndst1fl / fl Alb - Cre + mice (i.e., in mice expressing both LDLR and LRP1) when compared with ApoC - III — bearing TRLs (Figure 10).

Physiological differences between human and rat primary hepatocytes in response to liver X receptor activation by 3 -[3 -[N -(2 - chloro -3-trifluoromethylbenzyl)- (2,2 - diphenylethyl) amino] propyloxy] phe nylacetic acid hydrochloride (GW3965).

But while researchers have previously been able to infect cultures of human hepatocytes with HBV, the cells» limited lifespan has made it difficult to study the virus, says Bhatia, who is also a Howard Hughes Medical Institute investigator and a member of MIT's Koch Institute for Integrative Cancer Research and Institute for Medical Engineering and Science.

Leukemic cells can feed themselves through increased secretion of hepatocyte growth factor (HGF), which then activates its receptor, MET, to promote growth.

Quantification of HNF4α immunostainings showed that over 90 % of hiPS cell - derived hepatocytes differentiated using our protocol express HNF4α [4].

A primary hepatocyte from an obese mouse, showing close interaction between the endoplasmic reticulum (red) and the mitochondria (green).

A second intriguing possibility would be the use of hiPSCs to produce functional cells for use in extra-corporeal applications, such as mature hepatocytes for use in bio-artificial livers.

Anderson and Langer have previously developed nanoparticles, now in clinical development, that can deliver siRNA to liver cells called hepatocytes by coating the nucleic acids in fatty materials called lipidoids.

The researchers found that patients from MODY3 families had one of several different mutations in the gene for hepatocyte nuclear factor 1 alpha (HNF -1-alpha) but healthy subjects had normal copies of the gene.

Binding and uptake of isolated [3H] TRLs (50 μg / ml) from control ASO - and ApoC - III ASO — treated Ldlr — / — Ndst1fl / fl Alb - Cre + mice in primary hepatocytes isolated from Ndst1fl / fl Alb - Cre + and Ldlr — / — Lrp1fl / fl Alb - Cre — mice after a 4 - hour incubation at 37 °C (n = 3 / condition).

Coffee enema was given four times a day, for the first year as hepatocyte destruction was recognized.

HNF4α immunostaining showed over 90 % of hepatocytes differentiated with our protocol expressed HNF4α (Asplund et al. 2016).

They based their approach on a system they had previously developed for studying the hepatitis C virus, in which they were able to successfully infect human hepatocytes with the virus and use it to compare antiviral regimens.

ES derived hepatocyte differentiation on laminins represent significant improvement of metabolic activity and functional organisation

Selva, D. Hammond, G. Thyroid hormones act indirectly to increase sex hormone - binding globulin production by liver via hepatocyte nuclear factor - 4α.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.

Mitochondrial and cytoplasmic fractions were obtained from whole flies with Abcam Mitochondrial Isolation Kit for Tissue (ab110168) and from robin fibroblast and mouse hepatocyte cell lines with Abcam Mitochondrial Isolation Kit for Cells (ab110170).

When Lin engineered the telomerase - expressing hepatocytes to die in response to a chemical signal and gave the mice with a liver - damaging chemical, he found that those animals in which the telomerase cells had been killed exhibited much more severe liver scarring than those in which the cells were functional.

Current protocols for generating hepatocytes from hESCs are hampered by inefficient differentiation procedures that lead to low yields and large cellular heterogeneity.

Another characteristic used to evaluate hepatocyte models is the presence of functional alpha -1-antitrypsin (α1AT), a protease inhibitor produced in the liver.

An enzymatic defect in the tyrosine catabolism results in a toxic accumulation of byproducts within hepatocytes unless the mice are treated with a drug called NBTC.

Of the many types of polarized epithelia, we have focused on liver hepatocytes, which exhibit a complex polygonal polarity, and simple columnar cells of the intestine.

To address this, Lagasse has injected hepatocytes into lymph nodes in peripheral parts of a mouse's body — under the knee or arm — because that requires less invasive surgery.

«They are not doing all the «worker bee» functions of normal hepatocytes.»

The research also detected the proliferation of binucleate hepatocytes a sign that the liver is attempting to rectify damage caused by a low protein diet.

Many conventional hepatocyte cells that are transplanted to mice for in vivo testing last for only two or three days, but the drug and its various metabolites might take weeks to metabolize, so toxic effects might not be apparent in such testing.

Their livers also had less fat than either of the two soybean oil diets and showed no signs of hepatocyte ballooning.

Overexpression of variant PNPLA3 gene at I148M position causes malignant transformation of hepatocytes via IL -6-JAK2 / STAT3 pathway in low dose free fatty acid exposure: a laboratory investigation in vitro and in vivo.

When blood glucose levels are high, hepatocytes respond to insulin by increasing glycogen storage, decreasing gluconeogenesis, and decreasing glycogenolysis.

This protease activates hepatocyte growth factor (HGF) and urokinase plasminogen activator (uPA), two proteins thought to be involved in the growth and motility of cancer cells, particularly carcinomas, and in the vascularization of tumors.

Overall, the patient's clinical course was compatible with immune - mediated destruction of rAAV - transduced hepatocytes.

DESCRIPTION / MICROSCOPIC FINDINGS / COMMENTS: Microscopic Description: Cellularity is moderate to high consisting of clusters of well - differentiated hepatocytes which display vacuolar change consistent with lipid admixed with blood.

In contrast, Smc6 is not present in HBV - infected hepatocytes (red).

Many researchers are already growing hepatocyte - like cells: Bhatia, for example, has already commercialized a device that uses bioengineered cells for drug testing.

In vivo administration of lentiviral vectors triggers a type I interferon response that restricts hepatocyte gene transfer and promotes vector clearance.

Weltman MD, Farrell GC, Liddle C. Increased hepatocyte CYP2E1 expression in a rat nutritional model of hepatic steatosis with inflammation.

Ag - NPs coated with galactose and mannose were considerably less toxic to neuronal - like cells and hepatocytes compared to particles functionalized by glucose, ethylene glycol or citrate.

Further experimentation showed Lagasse that if he injected hepatocytes directly into the lymph nodes, the cells picked up signaling proteins (essentially SOS signals to grow) released into the bloodstream from the dying liver.

Rejection is not a problem because the animals were genetically engineered to share identical DNA, eliminating the risk that the immune system would attack foreign hepatocytes.

They found that hepatitis in these «human hepatocyte chimeric mice» was caused by white blood cells known as cytotoxic T lymphocytes (CTLs) that were specifically targeted to hepatitis B virus.

Eventually the liver can not generate new hepatocytes quickly enough to counteract the damage caused by the scar tissue and the organ fails.

«We think the combination of making more hepatocytes and reducing collagen is the most promising approach to treating liver fibrosis,» says Willenbring.

Holger Willenbring of the University of California, San Francisco, and his colleagues worked out a way to transform myofibroblasts into healthy hepatocytes using a cocktail of liver gene switches called transcription factors.