Sentences with word «supernatant»

T cell lines were maintained by biweekly stimulations in complete DMEM supplemented with 10 % prescreened FCS and 5 % conditioned medium that was prepared from culture supernatant of rat splenocytes stimulated with Con A for 48 h.

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Allantoic fluid and the cell culture supernatants from the first five passages were tested for hemagglutinating activity with a 1 % suspension of chicken erythrocytes to exclude avian hemagglutinating viruses (e.g., influenza virus and EDS - 76 virus).

The gaseous phase of cigarette smoke induced a 2 - to 36-fold increase in the activity of guanylate cyclase in supernatant and particulate fractions from various rat and bovine tissues over basal activity.

For each pool, 55 µL of rabbit sera and 55 µL of viral supernatant at a concentration of 100 TCID50 were mixed, incubated for 1 hour at 37 °C, and inoculated onto A549 cells in wells of a 96 - well plate as described above.

Antibody bound to breast cancer cells was detected by flow cytometry (Fig. 2C), and significant quantities of human IgG were detected on the surface of both HER2 - overexpressing cell lines following incubation with supernatant from HB1.F3 - H2IgG and HB1.F3.Ad - H2IgG NSCs.

To perform the virus neutralization assay, 55 µL of viral supernatant at a concentration of 100 TCID50 and 55 µL of serum (starting at a 1 ∶ 8 dilution) were mixed and incubated for 1 hour at 37 °C.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

His - tagged recombinant merozoite proteins were purified from spent tissue culture supernatants by using a custom - built, piston - driven, sample - loading apparatus as described previously [33].

Samples were centrifuged at 14,000 rpm for 3 minutes and supernatant transferred to clean tubes.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

Phosphorylation of supernatant protein factor enhances wits ability to stimulate microsomal squalene monooxygenase.

Virus - containing supernatants were collected forty - eight hours after transfection, passed through a 0.45 µm filter (Millipore) and concentrated by ultracentrifugation at 100,000 g for two hours at 4 °C.

«It's an interesting project because backwash supernatant disinfection is not widely used in Australia,» said Michael Bambridge, Managing Director of CST Wastewater Solutions.

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

Supplied as cell free supernatant from H9's or infected H9 cells.

The titer of ZIKV stocks were determined and 10-fold dilutions of the stock were used to study the sensitivity of the assay in a synthetic normal human plasma (Acrometrix, Benicia, CA), used in absence of naturally human infected ZIKV sample and Leibovitz 15 (L - 15) growth medium (GibcoBRL, Grand Island, NY, USA), used as supernatants of infected AP61 cells with ZIKV.

For those who do want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making an AAV1 supernatant.

Retroviral or lentiviral supernatants were collected and filtered 24 and 48 hours after transfection.

To remove hepatocytes, homogenates were centrifuged at 300 rpm for 3 min, and then supernatants were centrifuged at 1500 rpm for 10 min.

For the total input control, 20 % of the total supernatant was saved and frozen at − 80 °C.

The culture supernatants corresponding to every time point were used for IFNα concentration quantification.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

(A) Il22 expression was analyzed by real time RT - PCR (n = 5), (B) secreted IL - 22 in supernatants was quantitated by ELISA (n = 4) and (C) the percent cells producing IL - 22 (% IL - 22 +) was determined by intracellular cytokine staining (n = 3).

Virus was purified from crude viral supernatant using LentiX Viral concentrator (Clontech) according to manufacturer's instructions.

Red histograms on each graph show target cells incubated with supernatant from unmodified HB1.F3 NSCs as a negative control.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

1 ml of antibody - containing hybridoma supernatant is only $ 40, significantly cheaper than commercially available antibodies.

One example is SPF, or supernatant protein factor, which transfers substrate from an inactive to an active pool or from one enzyme site to another.

However, despite multiple serial passages, we were unable to propagate the infected cell culture supernatant in either BSC - 1 or PMK cells.

(c) Quantification of covered area by BP treated with supernatants of control (Ctr) or Ang2 - overexpressing (pAng2) HUVEC in a lateral scratch wound assay after 6.5 h normalized to control.

The supernatant recycling system installed at Bathurst Council's water filtration plant, left, is just one example of CST Wastewater's technology that will be showcased at Food Tech.

A major NSW regional council has introduced a supernatant recycling system into the filtration plant -LSB-...]

A major NSW regional council has introduced a supernatant recycling system into the filtration plant in the town water supply, helping to conserve potable water and providing substantial water savings over the long term.

Extracts are centrifuged at 3750 g and then 1.4 ml of supernatant is transferred to a polypropylene Eppendorf tube and centrifuged at 16100 g (benchtop micro centrifuge).

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

With the current designs of magnetic stands, these purifications can be problematic, often leaving carryover contaminants when the pellet is disturbed or when the supernatant is not completely removed.

Extracellular vesicles (EVs) such as exosomes (70 nm — 160 nm in diameter) and microvesicles (100 nm — 1,000 nm diameter) can be harvested from cell - culture supernatants and from all bodily fluids.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

The supernatants were discarded, and the tissue was resuspended in DMEM supplemented with 10 % fetal bovine serum, 2 - mm l - glutamine, sodium pyruvate, nonessential amino acids, and a vitamin solution (Life Technologies, Inc., Rockville, MD).

To confirm the generation of infectious virus, viral supernatants were quantitated by an end - point dilution assay.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells