Sentences with phrase «cell sorting»

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

To isolate single colony variants in a non-mutating background, the floxed AID expression cassette was excised by culturing the cells in CM containing 20 nM 4 - hydroxitamoxifen (SIGMA) for three days followed by two rounds of cell sorting for stabilization.

The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.

Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.

That we observe cell sorting in EB culture also provides direct evidence, albeit in vitro, for the differential adhesion model proposed for the resolution of early PrEn and PrEc in the mammalian blastocyst in this same paper [56].

Investigating the dynamics of cell sorting at compartment boundaries by real - time in vivo imaging

• NanoCellect Biomedical, a San Diego - based developer of microfluidic cell sorting technologies for cell - based assays, raised $ 10 million in series B funding.

The core facility offers assisted cell sorting services for characterizing, separating and purifying populations of particles as diverse as beads, micro-particles and cells.

In our study we invsetigated CD4 + T cells sorted from spleens of infected mice.

• NanoCellect Biomedical Inc., a San Diego, Calif. - based developer of microfluidic cell sorting technologies for cell - based assays, raised $ 10 million in Series B funding, including convertible notes.

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

The new method was described in a September 2014 publication in the scientific journal Analytical Chemistry, «Surface free energy activated high - throughput cell sorting.»

Research at Nagoya University on cell sorting used a microfluidic chip to prevent sample infection.

Barbara Stewart, executive director of the Association of American Cancer Institutes, also expects cutbacks in shared resources such as cell sorting machines and tissue banks.

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

We also thank Robert Karaffa and Sommer Durham for providing essential support with cell sorting and Dr. Ruben Donis (CDC) for his generous gift of ferret anti-H5 serum.

Together with Anna - Maria Divne, I am setting up and establish methods for single cell genomics, from single cell sorting, to whole genome amplification, target screening and sequencing library preparation.Why can you find me on this homepage?

Institutional needs for flow cytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's investigators.

CD4 + T cells sorted by immunomagnetic beads from splenocytes of animals tolerant to donor - type cardiac allografts were plated at 106 cells / well.

New research at Rockefeller University, published March 18 in Nature, reveals how cells sort out the loops meant to encode small RNAs, known as microRNAs, by tagging them with a chemical group.

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

I mastered various blotting techniques; performed complex, cell - based assays; and become skilled in the use of flow cytometry (FACS) technology to complete advanced cell sorting, cell counting and biomarker detection.

The work was also supported by the Genomic & RNA Profiling Core, Cytometry & Cell Sorting Core and Human Tissue Acquisition & Pathology Core at Baylor, and the Texas Advanced Computing Center at The University of Texas at Austin and Rice University resources.

In addition, Rubin pointed to the advent of single - cell sequencing with microfluidic and cell sorting approaches, focused specifically on cells that lack genes that match previously identified ones, as another approach in the search for completely novel organisms.

The enlarged view shows a demonstration of on - chip cell sorting of a Euglena gracilis cell (time interval of each frame: 40?

Notably for their analysis, the researchers employed a powerful cell sorting technique to allow them to study pure populations of heart muscle cells (cardiomyocytes) rather than a mix of all cell types in the heart — which, due to an alteration in composition during disease, would confound analysis.

Typical image of fused cell sorted by FACS and visualized by fluorescence microscopy.

Bruce Drinkwater, Professor of Ultrasonics in the Department of Mechanical Engineering and one of the authors of the study, said: «We have now shown that these vortices can rotate microparticles, which opens up potential applications such as the creation of microscopic centrifuges for biological cell sorting or small - scale, low - power water purification.

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

This Core provides flow cytometry and automated cell sorting services.

Amani Said (Dahmann, MPG)-- «Mechanisms underlying cell sorting at the A-P compartment boundary in the Drosophila wing» (2007)

Following gesicle treatment, CD81 - negative hiPS cells sorted via flow cytometry were counted using a hemocytometer.

Katharina Landsberg (Dahmann, MPG)-- «The role of cell bond tension in directing cell sorting at the anteroposterior compartment boundary in wing imaginal dices of Drosophila melanogaster» (2010)

Increased cell bond tension governs cell sorting at the Drosophila anteroposterior compartment boundary.

3D Flow ™ Analysis is a streamlined workflow that integrates standard cell sorting with NanoString nCounter ® analysis, thus enabling highly multiplexed digital detection of 770 RNAs and 30 proteins from a single sample.

To examine IL - 22 production specifically in ILCs, we isolated ILCs through cell sorting from Rag1 - / - mice.

From other papers I would like to highlight the one by M. Mehrshad and enigmatic SAR202 cluster, the one by A. C. Lehours and biogeographic patterns of aerobic anoxygenic phototrophic bacteria and the one by E. M. Llufrio and how fluorescence activated cell sorting introduces oxidative stress and alters the metabolic state of cells.

FACS - if you're knocking out a cell surface protein, then cell sorting can be used to rapidly isolate those clones that have lost the protein of interest

To test this we plated cells sorted by flow cytometry clonally and assessed the extent to which colonies could re-establish steady - state equilibrium.

Upon approval of your registration by the FMCF manager, you will be provided with a login name and password to access the Online Booking System, which will allow you to book cell sorting appointments.

W. E. Reintsch, A. Habring - Mueller, R. W. Wang, A. Schohl, F. Fagotto, beta - Catenin controls cell sorting at the notochord - somite boundary independently of cadherin - mediated adhesion.

After 20 rounds of preparative cell sorts, the mutational machinery was stopped by inducing the excision of the floxed AID gene which is necessary for SHM [10].

By combining advanced approaches such as cell sorting and single cell analysis, researchers will gain a better understanding of the complex genetic landscape of disease.

Cell sorting services are available using the AutoMACS platform housed in a class IIA biosafety cabinet.

At the University of Cologne, biophysicists in the lab of Professor Berenike Maier were now able to show how differential mechanical forces can lead to cell sorting in biofilms, thereby determining their architecture.

This simple protocol is based on automated fluorescent activated cell sorting (FACS) of OPCs.