Sentences with phrase «expressing cells»

Defined human cell line cores containing positive and negative protein expressing cell lines on the same slide

Conditioned medium from both GBP1 and GBP2 expressing cells induced lower levels of ILP2 and ILP5 accumulation than GFP conditioned medium.

The authors uncover the cellular and molecular mechanisms, as well as the gene network regulated by Sox9 during the early steps of skin tumor initiation and demonstrates that Sox9 controls the long term maintenance and expansion of oncogene expressing cells by promoting self - renewing division and inhibiting differentiation.

It was even more surprising to observe that in absence of Sox9, pre-cancerous cells disappear over time, suggesting that we can eliminate oncogene expressing cells before cancer formation» comments Jean - Christophe Larsimont, the first author of this study.

Negative, intermediate and / or strong protein expressing cell lines cores verified using Quantitative Digital Pathology (QDP)

Scientists have discovered that low levels of ATP make cancer gene expressing cells suicidal.

«That is why detecting early esophageal cancer with fluorescent probes to highly expressed cell surface proteins are necessary.»

Zhao, Chen and colleagues found that these gastrin - dependent peptide receptor - expressing cells also express a type of serotonin receptor known as the 1A receptor.

Most ILCs that produced IL - 22 also produced GM - CSF, but not all GM - CSF expressing cells produced IL - 22 (Fig 6B).

These inhibitors effectively targeted and blocked Msi expressing cells, resulting in halted tumor growth in animal models as well as in patient - derived cancer cells, which harbor more complex mutations and are uniformly drug - resistant.

To ensure that donor and host tissues could be easily distinguished, we generated EFTF - expressing cells from transgenic embryos constitutively expressing a variant of yellow fluorescent protein (Venus YFP)[10].

We then tracked the expression of CD45 and class I and class II molecules on HPC - derived cells in long - term chimeric Rag2γc − / − mice by gating for the GFP expressing cells.

The trimer - expressing cells even died more quickly than cells expressing mutant forms of SOD1 that are found in severe hereditary ALS cases.

The findings are consistent with previous studies suggesting that monoclonal antibodies in combination with chemotherapy work better in patients with EGFR expressing cells.»

The researchers found that, in test animals, modification of the blood - forming stem cells resulted in more than two years of stable production of CAR - expressing cells without any adverse effects.

In this immunofluorescence microscope image of cancer gene Myc expressing cells, active death protein Bak appears as green spots localizing on the surface of the mitochondria.

The team went on to show that supplementing heavy stable isotope - labeled forms of these precursors led to incorporation of heavy amino acids into proteins produced in enzyme expressing cells.

5 - HT3AGFP - expressing cells show increased intracellular Ca2 + in response to 5 - HT3 activation.

We showed previously that myostatin circulates in the blood and that systemic effects can be achieved by implanting myostatin - expressing cells into a single site in the lower limb [21]; however, there have been no experiments that have demonstrated conclusively that myostatin normally acts systemically.

GCSF and hypoxia inducible factor - 1α (HIF - 1α) have been shown to up - regulate SDF - 1α in the infarcted area in a rabbit ischemia model and promote the migration of CXCR4 expressing cells towards cardiac tissues [47 - 49].

Persomics now provides CRISPR based screening tools for CAS9 expressing cell lines, and we are currently working to extend this.

In addition, brains cultured with the conditioned media from GBP1 or GBP2 expressing cells revealed that diluted media induced ILP2 and ILP5 secretion more effectively.

In (D), data is reported as fluorescence intensity fold over DMSO - treated cells in each set of transfection *, p < 0.0001 compared to Bach1 - WT expressing cells at same compound doses.

The pairs of collagen expressing cells migrating toward each others until they touched at the midline.

The observation that p21 - / - and SV40 T - Ag - expressing cells behave identically with respect to life span extension as well as the high labeling index in the terminally nonproliferative state indicates that crisis is not a phenomenon induced solely by viral oncogenes, but a physiological state resulting from the bypass of normal senescence mechanisms.

In the converse test, 98 % of TdTomato expressing cells were GFP +, indicating a robust, but specific system.

Differentiation of this heterozygous iPSC line to erythrocytes used an improved feeder - free and xeno - free three - step culture strategy, generating enucleated hemoglobin expressing cells to a similar level to that observed for the parental TNC1 iPSC line (see figure).

FoxP3 expressing cells that were CD4 + were readily detected in tolerant allografts (Figures 7c — e).

This research shows for the first time that the retina has its own population of vasopressin - expressing cells which communicate directly to the SCN and are involved with regulating the circadian rhythm.

2013, advanced online), that includes data showing the endogenous GC response to SRBC is reduced in mice conditionally deficient for EBI2 in CD11c - Cre expressing cells.

Following uptake into target cells, conjugates made with the PEG4Mal linker were processed to a cytotoxic metabolite that was retained by MDR1 - expressing cells better than a metabolite of similar conjugates prepared with the nonpolar linker N - succinimidyl - 4 -(maleimidomethyl) cyclohexane -1-carboxylate (SMCC).

While this approach did not allow us to examine the impact of selective EBI2 - deficiency in DCs on WT B - lineage cells, the experiment did allow assessment of the response of EBI2 - deficient B cells in mice that lack EBI2 on CD11c + DCs while retaining EBI2 on approximately half of all non-CD11c expressing cells (such as helper T cells).

The fluorescent moiety of the BRET reporter was used to sort successfully infected / expressing cells by flow cytometry.

In the study, Stephen Maricich and colleagues identified a subpopulation of Atoh1 - expressing cells in hair and whisker follicles within mouse skin that exclusively give rise to Merkel cells during development and adulthood.

Unlike CD8 + DCs that express the cell surface protein CD205, CD8 — DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II.

The Rlm1 - expressing cells also became more permeable to large molecules during development.

Meanwhile, the ALPL - expressing cells produced on average more than twice as much bone matrix (and as much as nine times more in some trials) during three weeks of subsequent cultivation than a similar - sized population of unsorted adipose tissue cells and almost four times more bone matrix than cells that don't express ALPL.