Sentences with phrase «culture cells»

During this round compounds are tested in cultured cells or animals to find out how effective they are and whether they have any toxic effects.

To mimic the radiation treatment procedure, a batch of cultured cells were carried to radiation room in a similar way to radiation treatment groups but were not exposed to radiation.

The two French scientists cultured cells from lymph nodes of patients.

The experiment was on cultured cells, and the drugs were given at far higher doses than could be achieved in humans.

Furthermore, using cultured cells, they examined how the sex - related genes were regulated.

The researchers showed that the approach was successful both in tissue culture cells as well as in mice.

In contrast to other cultured cells, such tumor cells can divide indefinitely and a cell line can therefore be cultured for many years.

While all three reporters stain cultured cells and tissue sections, some reporters are better suited for particular applications.

A number of genes seem to regulate cell size and growth, but most of the work on these has been done in cultured cells.

Exactly how the genetic risk factors contribute to the disease is not readily apparent in most cases, and requires cellular and molecular biology investigations that use cultured cells and mouse models.

The blinking eye is created by culturing cells of the cornea and the translucent membrane of the inner eyelid on a 3D - printed scaffold shaped like a contact lens.

To find out, the researchers injected a cloned telomerase gene into cultured cells from retina, skin, and blood vessels, all of which are associated with degenerative, aging - related diseases.

Total RNA was extracted from cultured cells with RNeasy Mini kit (Qiagen), with the use of QIAshredder spin column for homogenization and an on - column DNase digestion.

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

The team then cultured the cells for several months in a bath of growth - promoting proteins, trying to get the cells to reverse course and become more like stem cells.

He presented his finding that mitochondrial dysfunction can induce senescence in cultured cells at the SENS6 Conference at the University of Cambridge.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

The team collected two small skin samples from each, and then cultured the cells in the lab and inserted the gene that makes fireflies glow into the gene that regulates the cells» clock.

The enzyme, called tankyrase, may prove useful for extending the lives of cultured cells grown to repair burned skin and other damaged tissue.

The CRISPR / Cas system is amazingly versatile and has proven to edit the genome effectively not only in mice and flies, but also in monkeys and, yes, in human cultured cells.

In fact, these dead starter culture cells and debris are an important food source for subsequent generations of microbes, referred to as non-starter lactic acid bacteria (NSLAB).

The virus kept turning cultured cells cancerous, and it kept causing tumors in animals.

However, there is minimal oversight of clinics that don't culture cells, because simple transplants are a «practice of medicine», which the FDA doesn't control.

The symptoms of Menkes disease arise because of a lack of copper in the body, although cultured cells lacking ATP7A actually accumulate copper.

Howden says the faster process also means the cell culture period is greatly reduced, potentially minimizing the risks associated with culturing cells outside of the human body, such as genome instability or other epigenetic changes.

(B) RT - PCR of nAChR subunit transcripts in cerebellar cultured cells and renal fibroblast cultures.

After successfully imaging cultured cells, the researchers tested their microendoscope on anesthetized mice.

To test this theory, they identified a short peptide that inhibits the binding of cyclin A-cdk2 to E2F and used it to treat cultured cells.

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They found high levels of the regulatory T - cells in patients treated with post-transplant cyclophosphamide, and lab - cultured cells survived cyclophosphamide treatment.

In the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implant.

Like an eager first - grader playing show - and - tell, she's been known to pass around a flask of cultured cells so that visiting scientific reviewers can see the raw data and share her excitement.

The researchers found that several lipidoids that had not performed well in cultured cells did deliver RNA efficiently in the zebrafish model.

For example, cultured cells exhibit rhythmic oscillations in gene expression where there is no input from the master clock.

A prototype device built by nScrypt, a company in Orlando, Florida, deposits one - hundredth - of - an - inch - thick dollops of lab - cultured cells onto a layer of gel - based paper invented at the University of Utah.

Small DNA - like molecules, called peptide nucleic acids (PNAs), can shut down specific genes in cultured cells because their nucleic acid sequence binds tightly to complementary target DNA or RNA sequences, preventing the cell from translating a gene's code into a working protein.

Nesson and his colleagues used a highly sensitive magnetometer to show that even the sterile media which are used to nourish cultured cells contain tiny particles of magnetite.

«I think it's going to become the standard by which everybody cultures their cells, including trying to develop conditions for human cells,» said Oatley.

Experiments with cultured cells showed that in the presence of group A Streptococcus, the nanosponges successfully acted as decoys, preventing toxins from reaching cells important for defense against infection, such as white blood cells and skin cells.

The new microfluidic approach is therefore a promising technique in tissue engineering, in which damaged tissue is repaired by using cultured cell material of the patient.

When the scientists administered an influenza virus dose of 107 TCID50 (a measure of the amount of virus required to produce cell death in 50 percent of cultured cells inoculated with virus) to 13 volunteers, 9 (or 69 percent) shed virus and developed symptoms.

Incubating cultured cells with RK - 33 had a similar effect, knocking down DDX3 expression in cells that highly express this protein and hampering their ability to multiply.

«However, if [electricity] can be successfully used to direct cultured cells to [form] a retinal or lens lineage, for example, it might have wide application in cell - replacement therapies,» he says.

Another option is to take a skin sample, culture cells called fibroblasts and freeze them.

«Our results suggest that megakaryocytes might be used clinically to facilitate adult stem cell regeneration and to expand cultured cells for adult stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.

The team first tested the antibody in cultured cells engineered to express the Nav1.7 sodium channel.

The researchers found that overproducing the microRNAs in cultured cells cut down the concentration of E2F1, a protein involved in a cancer growth pathway, suggesting that, in healthy cells, the microRNAs keep cell growth in check.

Additional experiments with cultured cells suggested that the mutation interferes with one of the enzymes that breaks down the APP protein and causes a 40 % reduction in β amyloid formation, Stefansson and colleagues report online today in Nature.